How Does Nucleus Pulposus Control The Annulus Fibrosus During Aging?
Poster Presentation Number: P392 Location: Venetian/Sands EXPO Hall D Spine Eric Mahoney Cincinnati OH Abubakar Atiq Durrani, MD Cincinnati OH Christopher Wylie, PhD Cincinnati OH Chitra Dahia, PhD Cincinnati OH Our study provides a spatial and temporal map of expression of cell signaling pathways in the mouse intervertebral disc. The aim of the study is to understand the cellular and molecular mechanisms by which the cells of nucleus pulposis (NP) influence those in the annulus fibrosis (AF). 8mm thick coronal and transverse cryosections, from lumbar vertebrae (LV) of 1-48 weeks old male mice were collected. Histology was analyzed by H&E and alkaline phosphatase staining. Cell proliferation and death was determined by phospho Histone H3 (PH3) and TUNEL staining, respectively. Immunolocalization of components of the TGFb, BMP, FGF and Shh pathways was carried out using confocal microscopy. During the first week, the AF became divided into a fibrous and mineralized component as determin
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