how does nuclear transfer work in mammalian embryos?
The principle is identical to what I’ve shown you in the frogs, but I’m going to show you a few movies to give you a sense of how it’s really done. So this is a microscope that’s set up for somatic cell nuclear transfer. And these things here are micromanipulators, including injection and suction devices that I’ll show you in a minute. I’m going to show you a series of three movies that were made by a very talent MIT graduate student, Kevin Eggan who is now a fellow over at Harvard, in Professor Rudolf Jaenisch’s lab, one of my colleagues over at the Whitehead Institute. And the first movie is going to be the isolation of nuclei from somatic cells. So here is a glass pipette, and Kevin is drawing up a cell into the pipette. And he is going to spit out, he has, you’ll see it again. He has spit out the contents of the cell other than the nucleus. So there’s this little thing in there that’s a nucleus. So here’s another cell. He’s pipetting it up and down to break it open and is retaining
