How does normalisation work?
Normalisation allows you to normalise the volume of the spots in your gels, allowing you to accurately compare the measurements of spots in different gels. There are two methods in Dymension: Total spot volume normalisation The raw volume of each spot is divided by the total raw volume of all the spots on the replicate image and multiplied by 100 to give a normalised volume percentage and this value is displayed in the Results table. Selected spot normalisation Each gel may be spiked with a known amount of a marker protein and therefore the resulting protein spot will be a consensus spot – it will appear in all the replicates and all the samples. Select this spot in any of the images and from the Normalisation menu choose Selected Spot. You will then be asked to enter a value and this can be any value, for example, 1000. The numbers for normalised volumes displayed into the Results table is calculated as follows: Spot raw volume / selected spot raw volume value x assigned value for sel
