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How does Midland convert OD units to nmol?

April 26, 2017convert midland nmol od units

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How does Midland convert OD units to nmol?

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The number of nanomoles is determined by dividing the total number of OD units by the molar extinction coefficient for the oligo as determined from the base composition of the sequence. Dividing the number of OD units by the length of the oligonucleotide, and multiplying this quotient by 100 can give a quick approximation for the number of nmol.