How does MicroVigene handle weak or negative spots?
When a spot signal is less than one standard derivation of the background, the signal is insignificant. MicroVigene gives you the option to set the minimal signal in unit of standard derivation. For example, if the value is set to 0.1, any signal below 0.1 of its standard derivation, will be reset to 0.1*(standard derivation). If it is set to a negative number, no minimal will be set.