How does KINAXO Cellular Target Profiling® work?
KINAXO determines a compound’s cellular target profile, including affinities, using sophisticated affinity-based separation procedures in combination with ultra-sensitive mass spectrometry. We use immobilized compounds to detect their protein binding partners from cell lines or tissue samples. The target affinities for unmodified, parental compounds are measured with quantitative mass spectrometry using stable isotope labeling of amino acids in cell culture (SILAC) or chemical labeling (iTRAQ /TMT). Co-crystallization data, homology models or SAR (Structure Activity Relationship) data support the choice of an appropriate site for immobilizing the client’s drug candidate. Naturally, we confirm that the linked compound binds and inhibits known targets just like the free inhibitor. KINAXO Cellular Target Profiling® services can easily be adapted to a broad range of small-molecule compounds. To date, we have successfully profiled dozens of structurally diverse and functionally unrelated ki
