How does Invitrogen use the in vitro Ki value to predict clinical relevance?
The ratio [I]/Ki is used to predict the likelihood of an in vivo interaction, where [I] is defined as the steady-state Cmax value for total drug (bound and unbound) following administration of the highest proposed clinical dose. We consider an estimated [I]/Ki ratio of greater than 0.1 to be positive, at which point a follow-up in vivo evaluation would be recommended.
