How does Flow Cytometry work?
Light scattered in the forward direction is collected by the Forward Scatter channel (FSC) photodiode and provides information on the relative size of the particle (see diagram below). Light measured at a 90° angle to the laser excitation beam is collected by the Side Scatter channel (SSC) photomultiplier tube (PMT) and provides information on the relative granularity of the particle.
Related Questions
- Are there special considerations I need to take into account when using the eFluorTM Nanocrystal conjugated antibodies for flow cytometry?
- What are the advantages of sorting by Flow Cytometry compared to other isolation techniques (i.e. magnetic bead separation, panning, etc.)?
- What are the policies of the Flow Cytometry Core?
