How does extrusion work?
A Liposome extrusion is a process in which multilamellar vesicles (MLV’s) are structurally modified to large unilamellar vesicles (LUV’s). Vesicles are physically extruded under pressure through polycarbonate filters containing pores of pre-determined pore sizes. The polycarbonate membranes are housed in a stainless steel Extruder which is easily assembled and disassembled. Every component of the Extruder can be autoclaved (see cleaning section below). The resulting lipid dispersion is a homogenous formulation consisting of unilamellar vesicles in which the mean vesicle diameter reflects the theoretical pore size of the membranes.
