How does embryology lab operate and what safety measure are taken ?
A. On the day before ooycte pick up media in which oocyte need to be kept in CO2 incubator for equilibration. Media for sperm preparation and for follicular rinsing are also kept for equilibration. • Percentage of CO2 in Incubator and temperature is checked with the fyrite kit and thermometer respectively. • The pH of the media & osmolarity is also checked. • Oocyte identification and grading are always done in laminar hood, under microscope having a hot plate with temperature 37°C. • Once the oocyte identification is done, it will be kept in special endotoxin free disposable petridish in special media. These petridishes are labeled with patient name and ART identification number. • A special needle is used to aspirate the follicle under ultrasound guided scanning machine. • The male partner are asked to collect the semen in a special container with his identification number. Semen is proceeded to get motile spermatozoa for insemination. • Embryology lab is a restricted entry area with
