How does DArT work?
Note: This technology is available for use to anyone that agrees to the improvement-sharing conditions of a royalty-free BiOS Genetic Resources Indexing Technology License. The process involves the development of a genotyping array. Genotypes for each DArT marker are then scored using hybridisation with the genotyping array. This can be done in a variety of different ways. In the most common implementation, the genomic representation fragments are stained with a dye that fluoresces green, while vector sequences are stained with a dye that fluoresces red, so that image analysis after the hybridisation, wash and scan can be done by comparison of the intensity of green and red fluorescence as in a typical expression microarray. The outcome is the genotype of the sample: a list of array features (spots) hybridising to the sample. The genotype is a table showing presence (1) or absence (0) for each marker. Any standard genetic analysis software can then be used to analyse the data. The firs
