How does AmpliTaq Gold® DNA Polymerase differ from AmpliTaq® DNA Polymerase?
AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase. This inactivation occurs as a result of the use of a proprietary chemical group, which binds to the active site of the enzyme. Removal of this chemical group, and thus activation of the enzyme, occurs during incubation at 95C. We recommend an initial activation step of 95C for 10 minutes when using GeneAmp™ 10X PCR Buffer and GeneAmp™ 10X PCR Buffer II and on an Applied Biosystems Thermal Cycler. If you are using the GeneAmp™ 10X PCR Gold Buffer, the initial activation time should be 95°C for 5 minutes. Each subsequent denaturing step, at 95°C, activates more of the enzyme, thus enzyme activation occurs throughout the thermal cycling reaction.
