How does adrenaline affect the heart rate of zebrafish?
EQUIPMENT Zebrafish embryos that are at least 36 hours old Medicine dropper Paper Towels Dissecting Microscope, Magnifying glass or Compound Microscope Depression Slide(optional) 3 Small Petri Dishes Adrenaline solutions in dropper bottles 0.0001% – 1ml needed per student group 0.00001% -1ml needed per student group 0.000001% – 1 ml needed per student group Stopwatch PROCEDURE 1. Read the lab thouroughly. Be sure you have read all of the questions that you will be responsible for answering with this lab. Be aware of what you are looking for. 2. Place the dish or slide under the microscope and find the embryo’s heart. It is the structure that is under the eye. Here is a diagaram to help you find it: 3. Designate who in the group will be the timer, counter and recorder. Timer: ________________________________ Counter: ______________________________ Recorder: _____________________________ 4. Practice counting the heart beat for 30 seconds. You should do this 3 times and alternate the acti
