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How does a gene specific shRNA or siRNA end up degrading the luciferase reporter in this system?

April 26, 2017degrading gene luciferase reporter shrna siRNA system

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How does a gene specific shRNA or siRNA end up degrading the luciferase reporter in this system?

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The shRNA/siRNA forms complex with DICER in the RISC to degrade the corresponding mRNA sequence to be targeted. This RNAi effect can be extended 5’ to the targetin g sequence into the luciferase mRNA, leading to the downregulation of luciferase protein activity.