How Do You Use Bacteria In Genetic Engineering?
Bacteria are useful tools in genetic modification. Through the use of plasmids, circular portions of interchangeable bacterial DNA, organisms, such as other bacteria, can be transformed genetically. This guide describes the basic methods involved in using bacteria for genetic engineering of another organism. Preparing the Gene of Interest Step 1 Obtain a sample of the first bacteria species by swabbing the enclosed colony with a sterile loop. Step 2 Wave the inoculated loop in a test tube through a water bath at approximately 48 degrees Celsius for five seconds. Step 3 Add a few milliliters of restriction enzyme to the test tube and heated, inoculated loop. This restriction enzyme varies depending on the gene of interest that will be transferred to the other bacterial species. Transforming the Other Bacteria Species Step 1 Dip a second sterile loop into a colony of the second bacteria species, the one that is to be transformed. Step 2 Spread the bacteria evenly across a Petri dish cont
