How do you use a transmission electron microscope
A transmission electron microscope is used to view the structure of a specimen at a nanometer resolution. The set-up and initial alignment of the microscope is usually done by skilled professionals. Knowledge that process is not necessary to use the microscope for an experiment. Essentially, the electron beam must be turned on and adjusted; the specimen inserted and brought into focus; and the image recorded for evaluation. Dim or turn off the primary lights in the room. Ensure that the attached computer is on and the attached vacuum system is running. Switch on the microscope by slowly turning the filament current knob clockwise. Do not go past the limit marked on the knob. Look at the imaging screen. Adjust the brightness of the image with the C2 knob until the resulting circle fills the entire screen. Select the option to create a small condenser aperture. Center the dot showing on the screen using the “shift x” and “shift y” knobs. This aligns two groups of lenses that focus the el
