How Do You Predict The Absorbance, For Without Using A Spectrophotometer?
edit: Salaam Scorpion I agree with the first answerer in part. Its ages since I have done any of this but you should have a straight line relationship and if you draw a line from the concentration (x axis) you are given in which you have to find an absorbance value (y axis) you just look across to the y axis and it will give you the value based on this straight line graph relationship. You should have an equation for the line of best fit so you could alternatively use that equation to predict the abosorbance value. It may not be a accurate predictor depending on how well your data are correlated (R2 value). Keep the concentration in mg/ml or you could use g/l (2mg/ml = 2g/l). But your tutor porobably wants you to keep as is. You cannot extrapolate beyond the line because you cannot predict how the data would go beyond that point….it is possible that absorbance v concentration does not have a linear relationship beyond the range you will have and thus plot. Do bare that in mind (the b
