How Do You Make Nutrient Agar For Petri Dishes?
Liquid nutrient broth is used to culture bacteria such as Escherichia coli. Recipes for this broth vary depending on the bacterial species and the presence of genetic modifications, e.g., antibiotic resistance. The broth can be solidified by adding agar, which enables the bacteria to form distinct colonies, whereas in liquid culture they simply disperse throughout the volume. This is a basic but essential technique for advanced methods such as gene cloning or microbiological assays. This article assumes that standard laboratory Escherichia coli strains are to be cultured on Luria broth (LB) agar plates (or petri dishes). Step 1 Weigh out 10 grams of bacterial-grade tryptone, 5 grams of yeast extract, 5 grams of sodium chloride, 15 grams of agar or agarose, and 1 milliliter of 1N sodium hydroxide. Mix these with a volume of distilled and autoclaved sterile water until 1 liter of medium is obtained. Autoclave the media in loosely capped bottles or flasks for 25 minutes. Allow to cool to
Liquid nutrient broth is used to culture bacteria such as Escherichia coli. Recipes for this broth vary depending on the bacterial species and the presence of genetic modifications, e.g., antibiotic resistance. The broth can be solidified by adding agar, which enables the bacteria to form distinct colonies, whereas in liquid culture they simply disperse throughout the volume. This is a basic but essential technique for advanced methods such as gene cloning or microbiological assays. This article assumes that standard laboratory Escherichia coli strains are to be cultured on Luria broth (LB) agar plates (or petri dishes). Weigh out 10 grams of bacterial-grade tryptone, 5 grams of yeast extract, 5 grams of sodium chloride, 15 grams of agar or agarose, and 1 milliliter of 1N sodium hydroxide. Mix these with a volume of distilled and autoclaved sterile water until 1 liter of medium is obtained. Autoclave the media in loosely capped bottles or flasks for 25 minutes. Allow to cool to approxi
