How do you make a restriction map for a circular plasmid?
That’s an interesting question. You should know what a restriction map looks like first: http://www.fgsc.net/plasmid/past30.gif. 1)You’ll start by drawing a small circle inside of a larger circle (use pencil and be prepared to redraw, it’s a bit messy to make one of these by hand). 2) At the top draw a straight line between the circles (like on the image link above). This line represents “zero” or a starting point. 3) Each cut represents the presence of 1 restriction enzyme sites. For example, PstI makes 6.8 and 5.9 fragments. That means that the two sites are located almost completely opposite of each other. There are 6.8 kilo bases (6,800 bases) between the sites on one side and 5.9 between them on the other. Draw those into your map. 4) Next look at HindIII. There are two sites located opposite from one another with 6.5 and 6.2 between them (note that when you add these up, it comes to 12.7… when you add up the P
