How Do You Make A Citric Acid Buffer Solution?
Chemical buffers prevent sudden changes in a solution’s acidity. Buffer solutions keep a certain pH level nearly constant during the course of biological reactions and are crucial for many physiological processes. All laboratory experiments in biochemistry or biophysics require buffer solutions. Citric acid is an organic acid that can efficiently maintain pH range from 3 to 6.2. In this example, you would make 200 ml of a citric acid buffer solution with the concentration 0.1 molar and pH 5.5. Step 1 Calculate the mass of citric acid needed to make the buffer solution using the following formula: mass(citric acid)=192.12 x molarity (solution) x volume (solution)/1,000 ml. (The molar mass of citric acid is 192.12 g/mole.) In our example, mass(citric acid)=192.12 g/mole x 0.1 M x 200 ml/1,000 ml= 3.842 g. Step 2 Weigh citric acid on the scale and place the chemical in the beaker. Pour the distilled water into the beaker. The volume of the water added should be about 10 ml less than the f
