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By Virginia B Gaces on November 22nd, 2009 After patient identification and preparation, the next vital step towards quality assurance is sample preparation. It is in this stage that specimens are carefully prepared depending on the substance that will be determined. Methods differ from one substance to another and these depend upon the intrinsic and extrinsic factors that affect the substance. Correct sample preparation is one of the factors that ensures reliable laboratory results; hence the vital contribution of this step should be reiterated. In the Clinical Chemistry section, the most common specimens used are serum and plasma and sometimes whole blood. Necessary skills to prepare these blood specimens should therefore be acquired by the laboratory technologist. A. SERUM PREPARATION: 1. Extract 5 ml of blood, or the needed volume, using venipuncture. 2. Remove the needle and dispense into test tubes. If vacutainer tubes are used, use the red top tube. 3. Allow to stand at room tem
After patient identification and preparation, the next vital step towards quality assurance is sample preparation. It is in this stage that specimens are carefully prepared depending on the substance that will be determined. Methods differ from one substance to another and these depend upon the intrinsic and extrinsic factors that affect the substance. Correct sample preparation is one of the factors that ensures reliable laboratory results; hence the vital contribution of this step should be reiterated. In the Clinical Chemistry section, the most common specimens used are serum and plasma and sometimes whole blood. Necessary skills to prepare these blood specimens should therefore be acquired by the laboratory technologist. These are the materials needed in the preparation of these blood specimens: 5 test tube, 13 x 100 applicator stick centrifuge machine vial with anticoagulant pasteur pipette aspirator test tube rack rubber stopper parafilm evacuated tubes A. SERUM PREPARATION: 1. E
