How Do You Design A Cell Line To Test For Anticancer Agents?
Plant Materials: Fresh plant materials were collected from the Forest Research Institute of Malaysia (FRIM), Kuala Lumpur, Malaysia. Plant extraction was conducted at the Microbiology Laboratory, Faculty of Applied Science, Universiti Teknologi MARA (UiTM) from July 2006 to December 2006. The anticarcinogenic activity of HepG2 and non-malignant Chang’s liver cell lines was conducted at Laboratory 4172 and 4176, School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM) from January 2007 to October 2008. Preparation of plant materials: Plant materials were dried in an oven at 50°C and then soaked three times in ethanol. The extracts were then filtered and concentrated under reduced pressure using a rotary evaporator (Buchi V-500) at 40°C. The concentrated ethanolic extract was dissolved in 1% DMSO to generate various concentrations of extract (1.56–200 μg mL-1) for further analyses. Cell culture: The human hepatoma (HepG2) and non-mal
