How do you deconvolute the results of a pooled shRNA experiment?
Deconvolution after a pooled shRNA experiment can often be the most challenging aspect of an experiment. We currently suggest one of the two following methods to deconvolute which shRNAs are having an effect in your screen. (1) Deep-Sequencing or Next Generation sequencing methods to identify which shRNAs are affecting your cell population, or (2) After selection with puromycin, pick colonies, expand clones and sequence verify the integrated shRNA. We are aggressively searching out new means for our customers to deconvolute.
