How do you calculate the extinction coefficient of an oligo?
RESPONSE:Calculate extinction coefficient of an oligo by either summing up the extinction coefficients of the individual bases times their number of occurrences. Or use a formula that takes into account nearest neighbor effects. algorithm for this calculation can be found on the web (http://www.basic.northwestern.edu/biotools/oligocalc.htmll). Just type in the sequence and the program will calculate the concentration of a 1 A260/ml solution. To calculate the MW of the aminomodified oligo just add 179.16 to the calculated MW of the unmodified oligo.
