How do TRANSIL membrane permeability assays compare to PAMPA?
PAMPA assays use various unstructured lipid mixtures on filter membranes. Since the lipids do not have the structure of true phospholipid bilayers, they cannot mimic the structure and charge interactions between the test substances and the membranes nearly as well as the TRANSIL assay kits. Also, non-specific binding confounds PAMPA measurements more strongly than TRANSIL assays. Filter membranes are well known to attract sticky compounds. This interaction with the filter membranes results in biased or even failed permeability estimates. PAMPA assays require overnight incubation while TRANSIL plates require only 2 minutes incubation time. PAMPA plates have to be prepared and validated in your laboratory, while TRANSIL plates are ready to use and validated by us.
