How do the modified amino acids work?
L-Photo-Leucine and L-Photo-Methionine are amino acid analogs of L-leucine and L-methionine that are endogenously incorporated into the primary sequence of proteins during synthesis and then activated by ultraviolet (UV) light to covalently crosslink within protein-protein interaction domains. The powerful method enables characterization of stable and transient protein interactions without using completely foreign chemical crosslinkers and associated solvents that can adversely affect the native environment.
