How do the GenePORTER Transfection Reagents work?
The positively charged primary amino functional groups in the polar region of the GenePORTER transfection reagents interact with the negatively charged phosphate backbone of the plasmid DNA, RNA or oligonucleotides electrostatically. The newly formed DNA/GenePORTER complexes have a net positive charge (when formulated under the recommended conditions), and will bind to the negatively charged plasma membranes of the cells. The complexes are subsequently taken inside the cells via endocyctosis.
