How do microarrays work?
Genetic material is extracted from a biological sample (for example, a whole blood or tissue biopsy sample). Segments of the genetic material that are pertinent to the biological query are amplified (or replicated) using Roche PCR technology. These replicated DNA segments, called amplicons, are labeled and applied to the microarray surface. The microarray is placed in a microfluidics chamber, where controlled temperatures promote the binding of the amplicons to complementary segments on the array. The resulting fluorescent pattern of hybridization is scanned and analyzed using sophisticated laser and computer technologies. For diagnostic applications, additional software algorithms are used to interpret the data (for example, translating genotype to predicted phenotype). Please note that the AmpliChip CYP450 Test does not have a claim for a tissue biopsy sample.
