How do I work out generation times of organisms using RABIT?
DETERMINATION OF MICROBIAL GENERATION TIMES USING RABIT Generation time of a microbial cell is the time taken for one cell to divide into two cells. It is also referred to as doubling time. Conventionally this parameter is determined from classical growth curves where numbers of organisms in a culture are counted over a time course and the corresponding generation time is determined from a graph of colony forming units against time. As Impedance microbiology is simply a measure of microbial growth it can be readily used to determine generation or doubling time without the need to construct growth curves from plate count data. Principle If a series of serial dilutions are prepared from an initial microbial culture then the generation time (tg) can be calculated by determining the delay in time to detection (TTD) between the respective dilutions. Using a known dilution factor, normally 1:100, it becomes unnecessary to even know the exact concentration of organisms in either dilution. Pro
