How do I use the GEArray to study gene expression?
GEArray is typically used to compare the expression of many genes between a control sample and one or more experimental samples using RNA prepared from cells previously treated under your experimental conditions. A simple single-tube enzymatic process converts the RNA into a biotin-labeled probe using a sample labeling kit specifically formulated for the arrays. The labeled sample then hybridizes with the nucleic acid crosslinked to the nylon membrane support. After washing excess non-specifically adherent label, a chemiluminescent detection method based on alkaline phosphatase (AP) conjugated to streptavidin illuminates the presence of the remaining label specifically hybridized to the microarray spots. All of the hybridization, washing, and detection steps are performed in the same disposable plastic hybridization tubes used to ship and store the arrays. Each spot on the microarray contains nucleic acid corresponding to a single gene. An accompanying Gene Table identifies the genes r
