How do I store oligos properly?
Even though it is convenient, we do not recommend storing oligonucleotides (in solution) at -20 degrees C. Repeated cycles of freezing and thawing can result in significant degradation of an oligonucleotide. Freezing and thawing occurs intentionally when an oligonucleotide stock is removed from the freezer and then returned after use. Unfortunately, unintentional cycles of freeze/thaw may also occur. The most common causes are frost-free freezers and temperature fluctuations in the freezer compartment. Changes in the storage temperature are commonly a result of frequent opening of the compartment. This is a particular problem for freezers used in common by several researchers.
