How do I store microsomes, S9, hepatocytes or media?
Media: (2° to 8° Celsius) Do not freeze media. Storing media refrigerated maximizes shelf life. Antibiotics: (-15° to -25° Celsius) Do not store in refrigerator. Avoid storage in “Frost-Free” freezer as temperature cycles can damage product. Microsomes/S9: (-70° to -90° Celsius) Store in mechanical cryogenic-freezer. Limit number of freeze/thaw cycles. Hepatocytes: (< -150° Celsius) Store in vapour phase of liquid nitrogen freezer. Do not allow product to thaw until ready for use. Do not store in liquid phase of liquid nitrogen freezer or Dewar type flask.