How do I set up standard concentrations with non-serial dilutions?
There are two methods for setting up non-serial dilutions or dilutions with a factor greater than 10. The following list shows a set of non-serial dilutions: • Standard 1 with concentration as 44500000 • Standard 2 with concentration as 4450000 (1:10 dilution) • Standard 3 with concentration as 2225000 (1/20 dilution) • Standard 4 with concentration as 1112500 (1/40 dilution) Automated method: Click the Standards button beside your standard assay to open the Set Up Standards pane, and then click Define Standards to open the Dilutions dialog box. Enter “4” for the number of points, 4450000 as the starting quantity, and 10 as the dilution factor. Back in the main Set Up Standards pane, directly type the correct values for the last two dilutions into the appropriate fields. Manual method: Click the Standards button beside your standard assay to open the Set Up Standards pane. Type 4450000 directly into the first field, and then press Enter to make the next field active. Type the second qu
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