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How do I sequence the amplification products from a real-time PCR run?

April 26, 2017amplification PCR products real-time run sequence

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How do I sequence the amplification products from a real-time PCR run?

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The UDSF staff has optimized a protocol for direct sequencing of real-time PCR products. Please refer to the specific section (“Real-time PCR sequencing”) in the Protocols.