How do I select the proper 129 strain to use as a control for my knockout created using a 129-derived ES cell line?
In order to choose the best 129 control strain for your knockout, you need to know what ES cell line was used to generate your knockout. The two references listed below contain useful information that can help to determine which 129 strain to use as a control. The Simpson et al. (1997) reference describes the history of 129 substrains and ES cell lines. Catalog numbers for 129 strains are available from The Jackson Laboratory are given as well. The Festing et al. (1999) reference lists the revised nomenclature for 129 substrains. Simpson EM, Linder CC, Sargent EE, Davisson MT, Mobraaten LE, Sharp JJ. 1997. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat Genet 16:19-27. [PubMed: 9140391] Festing MF, Simpson EM, Davisson MT, Mobraaten LE. 1999. Revised nomenclature for strain 129 mice. Mamm Genome 10:836.
