How do I screen pSMART clones for inserts?
In a typical ligation/transformation, there is no need to screen for inserts, since over 99% of the clones will have inserts. However, it is possible to use PCR with the SL1 and SR2 primers to confirm the presence or size of the inserts. PCR can be performed on the transformed colonies or on DNA prepared from the colonies. In addition, the cloning site of the pSMART vectors is flanked on both sides by EcoRI and EcoRV restriction sites, so a digest with either enzyme will excise the insert from the vector for gel analysis.
