How do I screen arrayed shRNA libraries?
When screening it’s important to have redundant shRNAs showing the same phenotype, thus mitigating the potential complication of off-target effects. Other RNAi providers may offer smaller libraries of pooled shRNAs targeting single genes. However, our research has shown that pooling at the gene level poses three potential dangers: (1) Muted functional or phenotypic effect, (2) Generation of a larger number of false positive leads and (3) Inability to rule out possible off-target effects.
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