How do I run multiple mRNAs against a single genomic sequence?
The entry in the mRNA textbox can be a single FASTA sequence, a single gi or accession, a list of FASTA sequences, or a list of gis or accessions. When multiple mRNAs are aligned to a single genomic sequence, the results page will start with a list of the mRNAs that were run so that you can easily jump down to see the results. You cannot mix FASTA sequences with gis or accessions. When you have multiple mRNAs, you can elect to produce a multiple alignment of those mRNAs against the genomic sequence. If the mRNAs overlap in any region, the multiple alignment will appear at the bottom of the results page. If the mRNAs do not overlap, you will see an error message. Why didn’t I get any results? There are a few reasons why you might not get any results. First, double-check the accessions/gis you entered to make sure that they are supposed to align. You may even want to use BLAST 2 Sequences to ensure that your sequences are related. Next, make sure that your mRNA sequences do not consist s
