How do I run a melting curve or dissociation stage at the end of a Relative Quantification (RQ) run?
The Relative Quantification (RQ) assay does not include a default dissociation stage setting. If you require a dissociation stage after an RQ assay immediately upon completion of a run, create an Absolute Quantification (AQ) project and program the thermal cycler settings to run the default dissociation stage only for your sample plate.
Related Questions
- What is a dissociation curve, and why is it particularly important to run a dissociation curve, following qPCR using SYBR Green chemistry?
- What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?
- Can I obtain Ct values from a Relative Quantification (RQ) run?
