How do I prepare the eFluor NC Flow Cytometry Staining Buffer?
eFluor NC Flow Cytometry Staining Buffer (cat. 00-3222) is supplied as a 5X concentrated stock solution. The 5X stock solution should be diluted with distilled water to a 1X solution. The eFluor NC Flow Cytometry Staining Buffer does not contain a source of protein and therefore, should be supplemented with 3% Fetal Bovine Serum (final concentration) or equivalent protein prior to use. The buffer should be stored at 4oC.
