How do I prepare agar plates for use with my spiral plater?
PREPARATION OF AGAR PLATES – SPIRAL PLATING Repetitive and predictable deposition of a solution on the surface of a prepared agar plate is a primary requirement of spiral plating. Although the Spiral Plater, when properly adjusted, will precisely deliver the volume from the stylus tip the amount and distribution of the sample on the surface of the agar surface is influenced by the condition and character of the agar surface itself. This is especially apparent on areas of the plate where the volume dispensed is very low and where factors other than the displacement of fluid by the syringe become operative, such as agar drag against the stylus tip. It is necessary that the agar surface be smooth without wrinkles, pits, or air bubbles; that the agar be of uniform depth over the entire plate; that no free water droplets are on the surface; that there are no areas of dehydration; and that there is no contamination on the plate from bacteria or mould. The following remarks on the consequence
