How do I plate the cryo-plateable hepatocytes for use with the Promega ADME-Tox assay kits?
The cryo-plateable hepatocytes should be plated following the Celsis IVT’s plating method. For CYP450 gene induction studies, cells are typically exposed for 1–3 days to inducers (Optimal length of treatment time should be determined empirically, though maximal inductions are typically reached after 48 hours of exposure to an inducer.). For determining metabolism with P450-Glo substrate, perform the P450-Glo™ Assay as described in Promega’s Technical Bulletin 325.
Related Questions
- Can you provide the general basis of the assay kits and how to calculate concentrations from the standard curve and sample data?
- With the P450-Glo assay kit, what plate format should be used to plate the cryo-plateable hepatocytes?
- How do I plate the cryo-plateable hepatocytes for use with the Promega ADME-Tox assay kits?
