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How do I overcome inadequate or poor transfer problems?

April 26, 2017inadequate OVERCOME poor problems transfer

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How do I overcome inadequate or poor transfer problems?

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Check to determine the size of the protein. If the protein is > 180 kDa, then you need to optimize transfer conditions by: • Using 20% MeOH. • Adding 0.05% SDS transfer buffer. • Increasing loading amounts of lysate. • Transfer for 1 hour at 1 A.