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How do I optimize my transfection procedure using the GenePORTER reagents?

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How do I optimize my transfection procedure using the GenePORTER reagents?

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First, it is important to be sure that the vector carrying the gene of interest has been optimized for high expression levels (e.g., phCMV and gWIZ vectors). If the vector has not been optimized, the level of gene expression could be minimal or none.

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