How do I optimize my PCR reaction for Real-Time PCR on the SmartCycler®System?
Information regarding the design of Real-Time PCR assays may be found in SmartNote 6.1 Designing Real-Time Assays on the SmartCycler®II System. Information regarding optimizing and analyzing Real-Time Assays on the SmartCycler® System may be found in SmartNote 6.2 Optimizing and Analyzing Real-Time Assays on the SmartCycler® II System.
Related Questions
- When using different miScript Primer Assays with the miScript PCR System, is it necessary to optimize reaction conditions for each miScript Primer Assay?
- What is the difference between a one-step and a two-step real-time PCR reaction?
- How do I optimize my PCR reaction for Real-Time PCR on the SmartCycler®System?
