How do I measure the binding of biotinylated molecules on streptavidin beads?
• Assay the supernatant for unbound molecules. This will determine the amount of molecule bound to the Dynabeads®. For nucleic acids, the concentration can be checked by OD-readings, or by running a gel. For proteins, the concentration in the supernatant can be determined by a spectrometer using a protein assay like BCA. Alternatively you can label the molecule with radioactivity or fluorescence and measure the concentration of molecule directly on the beads (former) or in the supernatant (latter).
