How do I measure an RNAi effect?
The most common way to measure gene specific knockdown is to perform western blot analysis to compare the level of protein expression before and after the introduction of siRNA. In some cases a reporter system that allows easy measurement of a reporter gene, such as b-galactosidase, may be used. Real-time PCR (such as by using LUX™ Primers ) to measure the level of transcript present in the cell or other types of cell-based assays may be also be employed.
