How do I learn to use LINEUP/ GelAssemble?
These are a bit more complicated to learn than is SEQED. To get a quick start, generate (for instance, with PILEUP) an .MSF file (or borrow one) and then run LINEUP. Enter command mode and issue the command HELP{return}. Read everything it says and then jump in and try editing the sequences. In addition to the commands that worked in Seqed, learn especially how to use the LOCK, UNLOCK, ANCHOR, and UNANCHOR commands (all in Command mode, the first pair controls the write locking of certain sequences, the second pair controls grouping of sequences – when two or more sequences are anchored together, edits and displacements apply to all sequences in the group.) LINEUP is not difficult to use, but to use it effectively you will have to learn a few common commands or you will spend half your time scanning the HELP for information.
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- How do I learn to use LINEUP/ GelAssemble?
