How do I know that the cleavage of peptide / tag was succesfull?
JPT Peptide Technologies validated the cleavage efficency for many different peptide surroundings. The efficiency of the cleavage can be checked by doing HPLC-runs after the cleavage was performed. The signal of the cleaved tag should bevisible at a retention time of 0 to 0.5min (C18, 3µ, 20x2mm, 1ml/min flowrate, 6 minute gradient, 5% to 95% Acetonitril water) and should light up when analyzed at 350nm. Uncleaved peptide /tag will have a signal at higher retention times (for examples and details about these validation steps, please contact our customer support).
JPT Peptide Technologies validated the cleavage efficency for many different peptide surroundings. The efficiency of the cleavage can be checked by doing HPLC-runs after the cleavage was performed. The signal of the cleaved tag should bevisible at a retention time of 0 to 0.5min (C18, 3µ, 20x2mm, 1ml/min flowrate, 6 minute gradient, 5% to 95% Acetonitril water) and should light up when analyzed at 350nm. Uncleaved peptide /tag will have a signal at higher retention times (for examples and details about these validation steps, please contact our customer support at peptide@jpt.com).
