How do I interpret what the Protein Probability Calculation chart is saying?
The protein probability calculation chart shows that using more peptides for protein probabilities gives a higher probability. In other words, the more peptides you have, the more confident in your protein probability you can be. Usually, this chart will tell you that even at a high peptide probability, using one peptide to ID a pritein will only allow for a relatively low protein probability, indicating that one hit wonders are not advised.